Comparing Patatin Class I and Camv 35s Promoters in Expression of Human Calcitonin Gene in Potato (Solanum Tuberosum Cvs. Kardal And Marfona)

Tissue Specific Expression of Human Calcitonin Gene in Potato Tubers by an Organ Specific Promoter

To increase the production level of heterologous proteins in plants, strategies such as choice of strongerpromoters, optimization of codon usage and specific localization of foreign proteins are of major concern.Calcitonin (CT), a 32 amino acid polypeptide is a powerful and specific inhibitor of bone resorption and isused to treat several human diseases. Calcitonin activity is...

Evaluation of polypyrimidine tract-binding protein gene promoters in potato (Solanum tuberosum cv. Désirée)

Transient Expression of Human Growth Hormone in Potato (Solanum tuberosum), Tobacco (Nicotiana tobacum) and Lettuce (Lactuca sativa) Leaves by Agroinfiltration

Using agro-infiltration technique, we have transiently expressed human Growth Hormone (hGH) in tobacco(Nicotiana tobacum), potato (Solanum tuberosum) and lettuce (Lactuca sativa) leaves. Out of three differentinoculation times used for infiltration in our study, it was seen that highest level of hGH expression wasachieved when leaves were infiltrated for 35 min. The presence o...

Comparison of hCMV immediate early and CaMV 35S promoters in both plant and human cells.

Cauliflower mosaic virus 35S promoter, widely used in transgenic crop plants, is known to be recognized in widely differing kinds of cells. Its activity in human cells may have impact on the risk assessment for the environmental release of genetically modified plants. In this study, transient expression of several constructs containing beta-glucuronidase (GUS) gene driven by cauliflower mosaic ...

همسانه سازی و بررسی بیان موقت پیشبر pat1 گیاه سیب زمینی با استفاده از سیستم اگرواینفیلتریشن

  To achieve the high level of gene expression, promoters are key elements. Patatin promoter has been used as a specific gene expression in potato tubers. Patatin is a group of glycol protein in potato tuber which is code by a multiple family genes. This protein makes up more than 40% of soluble proteins of potato tubers. Class I Promoters mainly responsible for tuber -specific expression patte...